016 Immune competent skin organoids reveal monkeypox dynamics

We have developed a model system that recapitulates nearly all elements of native human skin biology. Currently, the available systems to study biology are limited animal models or other incomplete in vitro culture systems. Animal suffer from well known differences between and organism biology, while fail incorporate critical such as stromal immune cells. Our newly contains skin, allowing for studies As assessed by histology, immunohistochemistry, single cell sequencing, reproduces near proportions cells make up epidermis dermis, including resident cells, fibroblasts, keratinocytes, many diverse types present skin. major barrier infection, must repel respond set stimuli. In first class proof principle we demonstrate active monkeypox infection within with correspondent viral cytopathic effects. Current work is aimed at utilizing this powerful tool increase our understanding virus dynamics associated response leads dissemination ultimately clearance.